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KMID : 0371819700020020073
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Nuclear Engineering and Technology
1970 Volume.2 No. 2 p.73 ~ p.84
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The Metabolism of(2-^(14)C) Mevalonic Acid on Photoperiodic Induction in Grafted Solanum andigena
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Bae Moo
E.I. Mercer
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Abstract
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The metabolism of sterol precurosor in leaves of Solanum andigena grafted between photoinduced and noninduced plant was investigated with the use of (2-14C) mevalonic acid. By the technique of the preparative gas-liquid chromatography, radioactive compounds of squalene, 4,4´-dimethylsterols and 4-demethylsterol were isolated and determined quantitatively. When labeled mevalonic acid was applied to leaves radioactivity was extensively incorporated into nonsaponifiable materials of lipid fraction and aqueous fraction (ethanol-water fraction). Radioactivity of 14C derived from (2-14C) mevalonic acid was transmissible from photoinduced plant to non-induced plant across the graft union, as tuberization hormone was, and incorporated into the sterols of the non-i ducQd plant. Inhibitors of sterol biosynthesis, SK & F 7997 and nicotinic acid, are effective suppressors of tuber growing, if applied to leaves during photoinduction period. The experimental results suggest that certain substance containing isoprene unit, or sterol-like compound may participate in tuber growing.
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